فصلنامه پاتوبیولوژی مقایسه ای
سال دوازدهم شماره 50 (زمستان 1394)

Salmonella is a member of the Enterobacteriaceae family. Infections in humans that are caused by these bacteria, are manifested asgastroenteritis, enteric fever (typhoid or para-typhoid) and septicemia. Class I integrons are the most common integrons found in the different serotypes of Salmonella enterica, that has caused multiple antibiotic resistance and problems in the treatment of infections resulted from these bacteria, in humans and animals. The aim of this study was to determine the prevalence of class I integrons in Salmonella enteritidis strains isolated from human and animal, and their antibiotic sensitivity or resistance. In this study, human)11 samples) and animal (13 samples) Salmonella enteritidis samples were received from the microbial collection of Islamic Azad University- Science and Research branch- faculty veterinary medicine . Then multiplex PCR experiments were performed to determine the frequency of class I integrons. Finally, agar disk diffusion method was used to determine antibiotic resistance pattern. The results showed that none of the 11 human Salmonella enteritidis strains had the class I integrons and 2 strains (15.3%) out of 13 animal Salmonella enteritidis had the class I integrons. As well 36.3% of human Salmonella enteritidis strains and 30.8% of animal Salmonella enteritidis strains showed multiple antibiotic resistance. In multiple-resistant strains that lacked class I integrons, resistance genes can be located on plasmids, transposons or other integron classes.

Aspergillosis is one of infectious disease that it causes difficulties for human and poultry. The main aim of this study is the diagnosis of Aspergillus parasiticus and flavus in forage samples by Duplex PCR method and comparison with culture and direct examination. After extracting DNAs of standard strains of Aspergillus parasitcus and flavus along with primers of each fungus and PCR testing, genes of each fungus were amplified and PCR product was cloned using TA cloning by pTZ57R plasmid. After optimizing Duplex PCR (D-PCR) monoplex PCR tests, 50 forage samples by Duplex PCR method, culture and direct were tested.In the optimized PCR test, 343bp and 413bp products of Aspergillus parasiticus and flavus were respectively amplified. Fifty samples of forage by Duplex PCR method, culture and direct were tested. 13 samples were positive only by Duplex -PCR and 15 samples by both tests direct and culturing were positive and 26 samples were negative with three methods. Results of McNemar's test is equal about the three methods tests for diagnosing Aspergillus parasiticus and flavus (p=0.824). Molecular methods such as D-PCR are faster techniques than direct testing and culturing for diagnosis of Aspergillus parasiticus and flavus but there arent difference between three methods for diagnosing Aspergillus parasiticus and flavus but with D-PCR we could diagnosis Aspergillus parasiticus.

Infectious bovine keratoconjunctivitis (IBK) is the most common infectious ocular disease of cattle throughout the world. In addition to Moraxella bovis as the principal causative agent, Infectious Bovine Rhinotracheitis (IBR) virus and Mycoplasma species probably act as risk factors for IBK. The objective of this study is to investigate the prevalence of mycoplasma sp. in the conjunctival sac of dairy cattle and its relationship with different stages of IBK. A total number of 60 samples from IBK-affected and healthy eyes were collected from different dairy farms during outbreaks of IBK and polymerase chain reaction (PCR) was employed to investigate the presence of mycoplasma sp. in the conjunctival sac. In this study, affected and healthy eyes consisted 61.8% and 38.2% of those with positive PCR results. As well, cases of conjunctivitis, keratitis and corneal ulcers consisted 23.8%, 33.3% and 42.9%, respectively. In conclusion, although there was no significant difference, greater percentages of positive PCR results were IBK affected and in addition consisted more severe forms of the disease. Therefore, Mycoplasma sp. infection may have a role in the pathogenesis and severity of IBK lesions.

A 7-month-old female Persian cat, 2.700 kg body weighting with a history of mandibular soft tissue swelling and an inability to open the mouth has been referred to private small animal clinic. The complications due to trauma to the jaw in three months and the lack of proper treatment, was created in clinical examination. The cat was disable to clean itself and softened canned food was eaten manually. The physical examination revealed the malocclusion of the mouth, the deviation of the mandible and atrophy of the right masseter musculature. Three-dimensional reconstructive CT and radiography were obtained. Under general anesthesia, surgical treatment was performed to remove the ankylosis block with the excisional arthroplasty of head of mandible. After surgery, the cat showed good functional use of the mandible to open its mouth and taking food and water without discomfort.

Any obstruction to urinary flow leads to obstructive nephropathy. The aim of this study was to evaluate the renoprotective effects of Crocin following unilateral ureteral obstruction in the rats. Forty male Wistar rats were randomly assigned into 5 equal groups. Groups 1 and 2 were considered as control and sham operated rats, respectively. In group 3 left ureter was obstructed surgically and in group 4 following the left ureteral obstruction, Crocin was gavaged at 50 mg/kg for 15 days. Finally, blood samples were collected for measurement of serum urea, acid uric and creatinine. Malondialdehyde and reduced glutathione contents for assessment of renal free radical activity; and enzymatic activities of glutathione peroxidase, superoxide dismutase and catalase as indicators of antioxidation, were measured in kidney homogenates. Histopathology of left kidney was conducted for verification of biochemical findings. Significant differences among the groups were determined by one-way analysis of variance followed by Tukey post-test. Statistical significance was considered at p

Avian influenza virus as a primary agent causes histopathologic lesions in infected chickens. Although, it was not any complete histopathologic study of ORT effects on chickens infected with avian influenza virus. The purpose of this study was to evaluate histopathological lesions induced by secondary infection with Ornithobacterium rhinotracheale on infected specific pathogen-free (SPF) chickens with H9N2 AI virus. Sixty, one-day-old SPF chicks were provided and divided randomly into three groups. At 21 days, the chicks in the group 1 were inoculated with H9N2 AI virus, group 2 was first infected by AI virus and 3 days later inoculated by ORT. Each bird in control group was inoculated with PBS. Then, the birds were euthanized and examined for histopathological lesions. The samples from various tissues were collected at 2, 4, 6, 8, 10, 12, 14 and 16 days post-inoculation (DPI). Histologic lesions in infected groups were observed as follow: congestion and hyperplasia of epithelium in trachea, pneumonia, hemorrhage and urinary tubules necrosis in kidneys, congestion of sinusoid spaces and portal vein in liver, lymphocyte depletion in the bursa of fabricius, hyperplasia of lymphoid Follicles in spleen, congestion and heterophil infiltration in thymus and increased of lymphoid Follicles in cecal tonsil. The results of this study showed that infected chickens by H9N2 AI virus under secondary infection with ORT cause increased histopathological lesions induced by virus.